<HTML><HEAD><TITLE>25º Congresso Brasileiro de Microbiologia </TITLE><link rel=STYLESHEET type=text/css href=css.css></HEAD><BODY aLink=#ff0000 bgColor=#FFFFFF leftMargin=0 link=#000000 text=#000000 topMargin=0 vLink=#000000 marginheight=0 marginwidth=0><table align=center width=700 cellpadding=0 cellspacing=0><tr><td align=left bgcolor=#cccccc valign=top width=550><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=3><font size=1>25º Congresso Brasileiro de Microbiologia </font></font></strong><font face=Verdana size=1><b><br></b></font><font face=Verdana, Arial,Helvetica, sans-serif size=1><strong> </strong></font></font></td><td align=right bgcolor=#cccccc valign=top width=150><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=1><font  size=1>ResumoID:2364-1</font></em></font></strong></font></td></tr><tr><td colspan=2><br><br><table align=center width=700><tr><td>Área: <b>Ecologia Microbiana ( Divisão I )</b><p align=justify><strong>਀䌀䄀刀䄀䌀吀䔀刀䤀娀䄀윀쌀伀 䤀一吀䔀刀 䔀 䤀一吀刀䄀ⴀ䔀匀倀䔀䌀촀䘀䤀䌀䄀 䐀䔀 䔀匀吀䤀刀倀䔀匀 䔀䰀䤀吀䔀 䐀䔀 刀䤀娀팀䈀䤀伀     
</strong></p><p align=justify><b><u>Juscélio Donizete Cardoso </u></b>  (<i>UEL</i>); <b>Douglas Fabiano  Gomes </b>  (<i>UEL</i>); <b>Maria Aparecida de  Matos </b>  (<i>IAPAR</i>); <b>Diva Souza  Andrade </b>  (<i>IAPAR</i>); <b>Mariangela  Hungria </b>  (<i>Embrapa</i>)<br><br></p><b><font size=2>Resumo</font></b><p align=justify class=tres><font size=2><meta http-equiv="Content-Type" content="text/html; charset=utf-8"><meta name="ProgId" content="Word.Document"><meta name="Generator" content="Microsoft Word 11"><meta name="Originator" content="Microsoft Word 11"><link rel="File-List" href="file:///C:%5CDOCUME%7E1%5CMICROB%7E1%5CCONFIG%7E1%5CTemp%5Cmsohtml1%5C01%5Cclip_filelist.xml"><!--[if gte mso 9]><xml>਀ 㰀眀㨀圀漀爀搀䐀漀挀甀洀攀渀琀㸀 
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<p class="MsoNormal" style="margin: 12pt 0cm 6pt; text-align: justify;"><span style="font-family: Arial;">No Brasil, três estirpes de <i style="">Rhizobium tropici</i> tipo B (<span style="">SEMIAs਀㐀　㜀㜀㴀䌀䤀䄀吀㠀㤀㤀Ⰰ 㐀　㠀　㴀倀刀䘀㠀㄀ 攀 㐀　㠀㠀㴀䠀㄀㈀⤀ 㰀⼀猀瀀愀渀㸀㰀猀瀀愀渀 猀琀礀氀攀㴀∀∀㸀☀渀戀猀瀀㬀㰀⼀猀瀀愀渀㸀猀漀 㰀猀瀀愀渀 猀琀礀氀攀㴀∀∀㸀愀甀琀漀爀椀稀愀搀愀猀 
para produção de inoculantes para a cultura do feijoeiro (<i>Phaseolus vulgaris</i>਀䰀⸀⤀⸀ 倀㰀⼀猀瀀愀渀㸀爀漀最爀愀洀愀猀 搀攀 瀀攀猀焀甀椀猀愀 瀀愀爀愀 猀攀氀攀漀 搀攀 攀猀琀椀爀瀀攀猀 搀攀 爀椀稀戀椀漀猀 琀洀 
buscado a eficácia e estabilidade das características da fixação biológica do਀渀椀琀爀漀最渀椀漀⸀ 倀漀爀洀Ⰰ 愀渀琀攀猀 搀攀 甀洀愀 愀瘀愀氀椀愀漀 搀愀 攀昀椀挀挀椀愀 搀攀猀猀愀猀 戀愀挀琀爀椀愀猀 攀洀 
experimentos de campo, uma caracterização inter e intra específica é quase uma਀攀砀椀最渀挀椀愀⸀ 一攀猀琀攀 猀攀渀琀椀搀漀Ⰰ 昀漀椀 爀攀愀氀椀稀愀搀愀 愀 挀愀爀愀挀琀攀爀椀稀愀漀 椀渀琀攀爀 攀 椀渀琀爀愀ⴀ攀猀瀀攀挀昀椀挀愀 
de 45 estirpes elite de rizóbios microssimbiontes de feijoeiro, pré਀猀攀氀攀挀椀漀渀愀搀愀猀 瀀愀爀愀 攀昀椀挀挀椀愀 猀椀洀戀椀琀椀挀愀⸀ 䘀漀爀愀洀 甀琀椀氀椀稀愀搀愀猀 㐀㔀 攀猀琀椀爀瀀攀猀 攀氀椀琀攀 ⠀䤀倀刀 㰀椀 猀琀礀氀攀㴀∀∀㸀倀瘀㰀⼀椀㸀ⴀ⤀ 瀀爀漀瘀攀渀椀攀渀琀攀猀 搀愀 䌀漀氀攀漀 搀攀 
Microrganismos de Interesse do Agronegócio do Laboratório de Microbiologia do਀匀漀氀漀 搀漀 䤀渀猀琀椀琀甀琀漀 䄀最爀漀渀洀椀挀漀 搀漀 倀愀爀愀渀⸀ 㰀猀瀀愀渀 猀琀礀氀攀㴀∀∀㸀☀渀戀猀瀀㬀㰀⼀猀瀀愀渀㸀倀愀爀愀 挀漀洀瀀愀爀愀爀 昀漀爀愀洀 㰀猀瀀愀渀 猀琀礀氀攀㴀∀∀㸀椀渀挀氀甀搀愀猀㰀⼀猀瀀愀渀㸀 愀猀 攀猀琀椀爀瀀攀猀 琀椀瀀漀 搀攀 㰀椀 猀琀礀氀攀㴀∀∀㸀刀栀椀稀漀戀椀甀洀 㰀⼀椀㸀焀甀攀 渀漀搀甀氀愀洀 攀昀攀琀椀瘀愀洀攀渀琀攀 漀 昀攀椀樀漀攀椀爀漀㨀 㰀椀 猀琀礀氀攀㴀∀∀㸀刀⸀㰀⼀椀㸀 㰀椀 猀琀礀氀攀㴀∀∀㸀琀爀漀瀀椀挀椀㰀⼀椀㸀 
A e B (CFN299, CIAT899); <i>R.</i> l<i style="">eguminosarium</i>਀戀瘀 㰀椀 猀琀礀氀攀㴀∀∀㸀瀀栀愀猀攀漀氀椀㰀⼀椀㸀 ⠀䄀吀䌀䌀㄀　　　㐀⤀㬀 㰀椀㸀刀⸀ 攀琀氀椀㰀⼀椀㸀 
(CFN42); <i>R. giardini</i> (H152); <i>R. galicum</i> (R602sp<span style="">) e, as SEMIAs 4080 e 4088<i>.</i> </span>As਀愀渀氀椀猀攀猀 搀攀 愀最爀甀瀀愀洀攀渀琀漀 挀漀洀 戀愀猀攀 渀漀 瀀漀氀椀洀漀爀昀椀猀洀漀 搀漀猀 瀀爀漀搀甀琀漀猀 搀愀 㰀猀瀀愀渀 猀琀礀氀攀㴀∀挀漀氀漀爀㨀 戀氀愀挀欀㬀∀㸀愀洀瀀氀椀昀椀挀愀漀 挀漀洀 瀀爀椀洀攀爀猀 㰀⼀猀瀀愀渀㸀搀漀 刀䘀䰀倀ⴀ倀䌀刀ⴀ㄀㘀匀 ⠀椀渀琀攀爀 
específica) e BOX A1R-PCR <span style="color: black;">(intra específica) das estirpes਀㰀⼀猀瀀愀渀㸀昀漀爀愀洀 爀攀愀氀椀稀愀搀愀猀 甀猀愀渀搀漀 漀 愀氀最漀爀椀琀洀漀 唀倀䜀䴀䄀 攀 漀 挀漀攀昀椀挀椀攀渀琀攀 搀攀 䨀愀挀挀愀爀搀⸀ 㰀猀瀀愀渀 猀琀礀氀攀㴀∀挀漀氀漀爀㨀 戀氀愀挀欀㬀∀㸀倀攀氀愀 琀挀渀椀挀愀 搀漀 倀䌀刀ⴀ刀䘀䰀倀 搀漀 ㄀㘀匀 爀䐀一䄀 瀀攀爀洀椀琀椀甀 愀最爀甀瀀愀爀 ㌀㐀 
estirpes IPR-<i style="">Pv</i> juntamente com as tipos਀焀甀攀 渀漀搀甀氀愀洀 漀 昀攀椀樀漀攀椀爀漀 愀氀洀 搀愀猀 㰀椀 猀琀礀氀攀㴀∀∀㸀刀⸀ 
tropici</i> (SEMIA4080 e 4088). Através da análise do dendrograma obtido por BOX-PCR,਀琀㰀⼀猀瀀愀渀㸀漀搀愀猀 愀猀 攀猀琀椀爀瀀攀猀 愀渀愀氀椀猀愀搀愀猀 愀瀀爀攀猀攀渀琀愀爀愀洀 瀀攀爀昀椀氀 搀椀猀琀椀渀琀漀Ⰰ 挀漀洀 攀砀挀攀漀 
de cinco pares de IPR-<i style="">Pv</i> que se਀愀最爀甀瀀愀爀愀洀 挀漀洀 ㄀　　─ 搀攀 猀椀洀椀氀愀爀椀搀愀搀攀⸀ 倀攀氀愀猀 愀渀氀椀猀攀猀 洀漀氀攀挀甀氀愀爀攀猀Ⰰ 瘀攀爀椀昀椀挀愀ⴀ猀攀 焀甀攀 
o BOX-PCR é discriminatório de estirpe caracterizando cada uma, enquanto, a do਀刀䘀䰀倀ⴀ倀䌀刀 ㄀㘀匀 刀一䄀爀  搀椀猀挀爀椀洀椀渀愀琀爀椀漀 搀攀渀琀爀漀 搀漀 最渀攀爀漀 攀 焀甀攀Ⰰ 攀砀椀猀琀攀 㰀猀瀀愀渀 猀琀礀氀攀㴀∀∀㸀☀渀戀猀瀀㬀㰀⼀猀瀀愀渀㸀愀氀琀愀 搀椀瘀攀爀猀椀搀愀搀攀 最攀渀漀琀瀀椀挀愀 渀攀猀猀愀 瀀漀瀀甀氀愀漀 搀攀 
estirpes pré selecionadas para eficácia na fixação biológica do nitrogênio.<o:p></o:p></span></p>਀ 
਀ 
</font></p><br><b>Palavras-chave: </b>&nbsp;BOX A1R-PCR, fixação biológica de nitrogênio, PCR-RFLP 16 S, Phaseolus vulgaris</td></tr></table></tr></td></table></body></html>਀�